Fig. 4

Signaling changes in HSPC subsets during hematopoietic recovery. Representative contour plots of A L⁻S⁻K⁺ and B LSK subsets after different days of 5-FU treatment (n = 4 mice in each group). Bar plots showing the frequencies of A CMP, GMP, MEP and B LT-HSC, MPP, and LMPP in the indicated cell populations after different days of 5-FU treatment. p values were calculated with Student’s t test. C–H Bar plots showing the frequencies of the indicated protein positive cells in C LT-HSC, D MPP, E LMPP, F CMP, G GMP, and H MEP cells from mice after different days of 5-FU treatment. p values were calculated with a one-way ANOVA followed by Tukey’s multiple comparisons test. n = 4 mice in each group. *p < 0.05, **p < 0.01, ***p < 0.001