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Fig. 13 | BMC Biology

Fig. 13

From: Large-scale data analysis for robotic yeast one-hybrid platforms and multi-disciplinary studies using GateMultiplex

Fig. 13

Procedure of GateMultiplex operation. The operation of GM_Basic and GM_Advanced can be divided into three parts, including input file information (in red background), cutoff setting (in yellow background), and output (in green background). The black dots under reference cutoff and the output file represent the numbers of the options. In GM_Basic, the operation starts from an input file, which would be converted into the required data format. The data would then be respectively analyzed by two cutoffs, reference cutoff, and background noise cutoff. The results from two cutoffs would be combined and further processed by tech-/bio-cutoff. The final results would be outputted as the result files. The operation of GM_Advanced is similar to that of GM_Basic. The blue words and dots in GM_Advanced represent the additional cutoffs or options. Internal control cutoff exerts before reference cutoff, and reference cutoff contains two more options (see Additional file 2 for the details). After being analyzed by tech-/bio- cutoff, the data could further be processed by positive cutoff. In the output file, one more file option is available

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