Fig. 2

SampleName, Treatment and Signal. a An Y1H screening example of a simple TF-prey batch containing 23 TF-preys (named from TF#01 to TF#23) and 1 negative control (shown as N in this figure). The TF name and the N are defined as “SampleName”. b–g The TF-prey batch would be mated with b DNA-bait(α), c DNA-bait(β), and d DNA-bait(γ), which would respectively result in the yeast cells with both e TF-prey and DNA-bait(α), f TF-prey and DNA-bait(β), and (g) TF-prey and DNA-bait(γ). DNA-bait is defined as “Treatment,” and different DNA-baits represent different “Treatment” types. h The yeast cells would grow into colonies and be further quantified into colony size values as “Signal”