Fig. 5

Reference cutoff. a One TF-DNA combination would be composed of 4 biological replicates (from bio-rep#1 to bio-rep#4). Each biological replicate is composed of 4 technical replicate colonies (from tech-rep#1 to tech-rep#4), resulting in 16 replicate colonies. b The 16 colonies in pink dashed frames belong to an experimental sample, and the other 16 colonies in blue dashed frames represent the reference sample. Through the black-and-white image, the size of each colony can be quantified. c The 16 colonies of reference were ranked based on the sizes. The size range from the 5th to the 12th (in blue background) were averaged. d The averaged value was defined as 1, and the fold-change value was set as 2 folds of the averaged value. As a result, reference cutoff includes the averaging ranges of the reference and the fold-change values. If the relative size of one experimental colony is larger than the cutoff value 2, then the colony would be regarded as a positive. If not, then the colony would be regarded as a negative.