Fig. 6

Biological and technical replicate cutoff. A TF-DNA combination contains 16 colonies as their biological and technical replicates. The 16 replicates would be processed into positives or negatives. Positive and negative replicates are shown as gray dots and empties, respectively. If biological and technical replicate cutoff is set as 8 replicates. The TF-DNA combination with more than or equal to 8 positive replicates would be identified as a positive TF-DNA interaction event. For examples, 4Bio-16Tech (as 16 positive technical replicates from 4 biological replicates), 4Bio-12Tech, 3Bio-12Tech, and 2Bio-08Tech represent the positive TF-DNA interaction events. Instead, the TF-DNA combination with less than 8 positive replicates would be regarded as a negative interaction event, such as 2Bio-07Tech, 1Bio-04Tech, 4Bio-04Tech, and 0Bio-00Tech