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Table 1 The comparison of GateMultiplex to two most widely used software

From: Large-scale data analysis for robotic yeast one-hybrid platforms and multi-disciplinary studies using GateMultiplex

  Language GUIa Input file format Adjustable parameters to identify
positive events
Output files Application
GateMultiplex C++ (fastest) Yes Format-free (1) Background noise cutoff
(2) Reference cutoff
(3) Bio/Tech replicates cutoff
(4) Internal control cutoff
(5) Positive cutoff
(1) Result file
(2) Fold-change file
(3) PNE file
(1) Yeast one-hybrid
(2) Yeast two-hybrid
(3) Drug development
(4) Precision agriculture
(5) Deep-sea fishery
SpotOn Perl NA HDF plate format (1) Reference cutoffb (1) Result file (1) Yeast one-hybrid
(2) Yeast two-hybrid
TIDY Matlab NA HDF plate format (1) Reference cutoffc
(2) Bio/Tech replicates cutoffd
NAe (1) Yeast one-hybrid
  1. aThe users can operate the GUI without further installing additional programming language user interface
  2. bNamed as z score cutoff in the original paper. The negative control value could be adjusted, but the fold-change value is not adjustable
  3. cNamed as background threshold in the original paper with the function similar to reference cutoff in this article. The fold-change value could be adjusted, but the negative control value is not adjustable
  4. dNamed as uniformity coefficient in the original paper with the function similar to Bio/Tech replicates cutoff. Only technical replicates can be processed but not biological replicates
  5. eThe output results only showed on the command lines