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Table 1 Datasets generated in this study and the results of barcoding using ONTbarcoder at 200X coverage (Consensus by Length) and 100X coverage (Consensus by Similarity)

From: ONTbarcoder and MinION barcodes aid biodiversity discovery and identification by everyone, for everyone

Dataset name Flow cell details
Run time/Guppy version
Raw reads/reads passing length threshold/reads of suitable length/ demultiplexed Demultiplexing rate/# QC_compliant barcodes/# Filtered barcodes with 1 N/# Filtered barcodes with > 1 N /# Unreliable barcodes
MinION R10.3 datasets
 Mixed Diptera
(658 bp, N=511)
R10.3: reused flow cell: 71 pores according to QC, but 500+ active during run
Runtime: 27.5 h
Guppy: 4.2.3+f90bd04
3,864,000/3,425,357/3,560,389/1,544,758 43.39%/495/2/5/8
Total success rate= 502/511 (98.2%)
 Afrotropical Phoridae
(658 bp, N=4275)
R10.3: new flow cell:
QC: 1101 pores
Runtime: 49.5 h
Guppy: 4.0.11+f1071ce
6,838,903/5,465,164/5,474,306/2,681,029 48.97%/3722/121/59/247
Total success rate= 3905/4275 (91.3%)
 Palaearctic Phoridae
(658 bp, N=9932)
R10.3: new flow cell:
QC: 1239 pores
Runtime: 47.5 h
Guppy: 4.2.3+f90bd04
16,595,984/15,658,174/16,100,505/5,012,489 31.13%/8026/108/231/780
Total success rate= 8365/9932 (84.2%)
 Palaearctic Phoridae
(313 bp, N=9929)
R10.3: new flow cell:
QC: 1297 pores
Runtime: 37 h
Guppy: 4.2.3+f90bd04
13,690,869/13,221,764/10,366,455/12,983,260/2,015,135 15.52%/8705/118/112/899
Total success rate= 8935/9929 (90%)
Flongle datasets
 Mixed Diptera Subsample
(658 bp, N=257)
Flongle: new
QC: 81 pores
Runtime: 24 h
Guppy: v 4.0.11+f1071ce
294,896/222,189/190,952/33,270 17.42%/185/35/20/9
Total success rate= 240/257 (93.4%)
 Chironomidae
(313 bp, N=191)
Flongle: new
QC: 74 pores
Runtime: 15 h
Guppy: 4.2.3+f90bd04
560,062/525,087/504,621/108,574 21.52%/178/1/2/6
Total success rate= 181/191 (94.8%)