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Table 6 Alternative DNA extraction methods

From: ONTbarcoder and MinION barcodes aid biodiversity discovery and identification by everyone, for everyone

Commonly used alternative DNA extraction methods Advantages Disadvantages
“directPCR”: “contaminating” a PCR reaction with the DNA of the target organism by adding the entire specimen or a tissue sample into the PCR reagent mix (Wong, Tay et al. 2014). • No cost
• No waiting time obtaining for template
• Time-consuming when subsampling is needed (antenna, leg)
• Low success rate for heavily sclerotized specimens
• No DNA template left after PCR
Commercial DNA extraction buffers: e.g., QuickExtract: 10 μl sufficient for obtaining DNA template from most insect specimens (Srivathsan, Hartop et al. [35]) • Long shelf life of buffers
• Template stays viable for weeks
• Additional DNA can be obtained through re-extraction of specimen
• Moderate costs (< 0.20 USD)
• DNA in leftover templates degrades within weeks/months
Commercial DNA extraction kits: e.g., DNeasy Blood & Tissue Kits • Template is stable • High cost (> 1 USD)
• Time-consuming