Fig. 6
From: Competition-based screening helps to secure the evolutionary stability of a defensive microbiome
Pairwise competition experiment, scoring the frequency of producer wins. A Representative images of agar plates at 5 days post-inoculation showing examples of the three competitive outcomes: win (producer S2 vs. non-producer St3 on Ps2 media), loss (producer S8 vs. non-producer St3 on control media), and draw (producer S2 vs. non-producer St3 on control media) (strain details in Additional file 1: Table S1). B Bar charts of competitive outcomes for the two Streptomyces producer strains (S8 and S2; Additional file 1: Table S1). Each Streptomyces strain was individually competed against ten different non-antibiotic-producer strains. Streptomyces is more likely to win on Pseudonocardia-infused media. A general linear mixed-effects model, with ten non-antibiotic-producer strains as a random intercept, was used to test for the effect of medium (control vs. Ps-infused) on the competitive outcome (win vs. loss). For analysis, draws were omitted. S8 on Ps1-infused media: nPs1-infused = 34, nControl = 95, χ2 = 103.6, df = 1, P < 0.0001; S2 on Ps2-infused media: general linear mixed-effects model, nPs2-infused = 50, nControl = 44, χ2 = 87.9, df = 1, P < 0.0001. lme4::glmer(outcome ~ medium + (1 | non-producer strain), family = binomial)