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Fig. 1 | BMC Biology

Fig. 1

From: Control of vein-forming, striped gene expression by auxin signaling

Fig. 1

ATHB8 Function in Vein Network Formation. (AJ,LU) Dark-field (AJ) or Differential-interfering-contrast (LU) illumination of cleared first leaves 14 days after germination (DAG). Top right: genotype. (K) Cardinality, connectivity, and continuity index (mean ± SE) as defined in [20] and Methods; cyclicity index (mean ± SE) as defined in Methods. Test genotypes were compared with the reference genotypes represented by the lines under the bars. Each index in athb8-11, athb8-27, SHR::miR165a, SHR::mATHB8:EAR, and SHR::mATHB8 was compared with the respective index in WT. Each index in SHR::miR165a;athb8-11 was compared with the respective index in SHR::miR165a. Each index in SHR::mATHB8:EAR;athb8-27 was compared with the respective index in SHR::mATHB8:EAR. Each index in MP::ATHB8 was compared with the respective index in SHR::mATHB8. Each index in MP::mATHB8 was compared with the respective index in MP::ATHB8. Difference between athb8-11 and WT cardinality indices, between athb8-27 and WT cardinality indices, between SHR::miR165a and WT cardinality indices, between SHR::miR165a;athb8-11 and SHR::miR165a cardinality indices, between SHR::mATHB8:EAR and WT cardinality indices, between SHR::mATHB8:EAR;athb8-27 and SHR::mATHB8:EAR cardinality indices, between SHR::mATHB8 and WT cardinality indices, between MP::ATHB8 and SHR::mATHB8 cardinality indices, between MP::mATHB8 and MP::ATHB8 cardinality indices, between SHR::miR165a and WT continuity indices, between SHR::mATHB8 and WT continuity indices, between SHR::miR165a and WT connectivity indices, between athb8-11 and WT cyclicity indices, between SHR::miR165a and WT cyclicity indices, between SHR::miR165a;athb8-11 and SHR::miR165a cyclicity indices, between SHR::mATHB8:EAR;athb8-27 and SHR::mATHB8:EAR cyclicity indices, between SHR::mATHB8 and WT cyclicity indices, between MP::ATHB8 and SHR::mATHB8 cyclicity indices, and between MP::mATHB8 and MP::ATHB8 cyclicity indices was significant at P < 0.05 (*), P < 0.01 (**), or P < 0.001 (***) by F-test and t-test with Bonferroni correction. Sample sizes: WT, 58; athb8-11, 39; athb8-27, 32; SHR::miR165a, 51; SHR::miR165a;athb8-11, 64; SHR::mATHB8:EAR, 38; SHR::mATHB8:EAR;athb8-27, 28; SHR::mATHB8, 33; MP::ATHB8, 37; MP::mATHB8, 47. (LU) Details of the upper fourth of the midvein. Arrowheads indicate gaps in xylem differentiation. Scale bars: (A,I,J) 0.5 mm; (B,C,F,G,H) 1 mm; (D,E) 0.2 mm; (LU) 50 μm

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