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Fig. 4 | BMC Biology

Fig. 4

From: Control of vein-forming, striped gene expression by auxin signaling

Fig. 4

MP Activity, ATHB8 Expression Domains and Levels, and Vein Network Formation. (AG,JN,PS) Top right: genotype. (DG,M,N) Bottom left: treatment. (AG,PS) First leaves 3.75 (F) or 4 (AE,G,PS) DAG (for simplicity, only half-leaves are shown in F and G). Confocal laser scanning (AG,P,Q,S) or differential interference contrast (R) microscopy. Dashed white line: leaf outline. (AG) ATHB8::nYFP expression (look-up table — ramp in H — visualizes expression levels). (I) ATHB8::nYFP expression level per cell expressed as mean gray value ± SE, ATHB8::nYFP expression domain length expressed as mean number of cells ± SE, and ATHB8::nYFP expression levels per domain expressed as mean gray value ± SE. Difference between bdl and WT, between MP::VP16:bdlΔI;iaa12-1;tpl-1 and WT, between MP::MPΔPB1:GR 3.75d -Dex → 0.25d +Dex and MP::MPΔPB1:GR 4d -Dex, and between MP::MPΔPB1:GR 4d +Dex and MP::MPΔPB1:GR 4d -Dex was significant at P < 0.05 (*) or P < 0.001 (***) by F-test and t-test with Bonferroni correction. Sample sizes: 26 (WT), 27 (bdl), 27 (MP::VP16:bdlΔI;iaa12-1;tpl-1), 18 (MP::MPΔPB1:GR 4d -Dex), 27 (MP::MPΔPB1:GR 3.75d -Dex → 0.25d +Dex), or 19 (MP::MPΔPB1:GR 4d +Dex) leaves; 265 (WT), 199 (bdl), 338 (MP::VP16:bdlΔI;iaa12-1;tpl-1), 248 (MP::MPΔPB1:GR 4d -Dex), 284 (MP::MPΔPB1:GR 3.75d -Dex → 0.25d +Dex), or 269 (dex-grown MP::MPΔPB1:GR) vein cell nuclei, and 316 (MP::VP16:bdlΔI;iaa12-1;tpl-1) or 608 (MP::MPΔPB1:GR 3.75d -Dex → 0.25d +Dex) flanking cell nuclei. (J-N) Dark-field illumination of cleared first leaves 14 DAG. (O) Cardinality index, connectivity index, and continuity index (mean ± SE) as defined in [20] and Methods; cyclicity index (mean ± SE) as defined in Methods. Difference between bdl and WT cardinality indices, between BDL::bdl:YFP and WT cardinality indices, between bdl and WT continuity indices, between bdl and WT connectivity indices, and between bdl and WT cyclicity indices, was significant at P < 0.05 (*) or P < 0.001 (***) by F-test and t-test with Bonferroni correction. (JS) Sample sizes: WT, 30; bdl, 65; MP::VP16:bdlΔI;iaa12-1;tpl-1, 22; MP::MPΔPB1:GR, 42; dex-grown MP::MPΔPB1:GR, 38; BDL::bdl:YFP (O), 20; BDL::nGFP, 46; BDL::BDL:GFP, 24; BDL::bdl:GUS, 32; BDL::bdl:YFP (S), 39. Scale bars: (AG,PS) 25 μm; (J,M,N) 1 mm; (K) 0.25 mm; (L) 0.5 mm

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