Fig. 3.
From: Neuroligin-1 mediates presynaptic maturation through brain-derived neurotrophic factor signaling
BDNF and NL1 increase the lifetime of AZs. A Cultured hippocampal neurons treated with buffer (-BDNF) or BDNF (+BDNF) on DIV4, treated with DMSO (control) or Anisomycin (+Anisomycin) on DIV5, fixed 6 h later and immunostained for bassoon (magenta) and MAP2 (green). B Quantification of the number of bassoon puncta per 10 μm dendrite for the conditions indicated in panel A. Mean + SEM; N = 3 experiments, n = 10 cells per experiment and condition; two-way ANOVA with post hoc Sidak tests: interaction is significant ***p=0.0001, F(1, 116)=99.82; ***p< 0.0001 for NT control vs. NT + Anisomycin; p> 0.05 for BDNF control vs. BNDF + Anisomycin. C DIV5 cultured hippocampal neurons transfected with mOrange or mOrange-tagged NL1 (green), treated with Anysomicin or DMSO for 6 h, fixed and immunostained for bassoon (magenta). D Quantification of the number of bassoon puncta per 10 μm dendrite for the conditions indicated in panel C. Mean + SEM; N = 3 experiments, n = 10 cells per experiment and condition; two-way ANOVA with post hoc Sidak tests: interaction is significant (***p< 0.0001, F(1, 116)=82.33; ***p< 0.0001 for mOrange control vs. mOrange + Anisomycin; p> 0.05 for NL1 control vs. NL1 + Anisomycin). Scale bar is 10 μm