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Fig. 4. | BMC Biology

Fig. 4.

From: Neuroligin-1 mediates presynaptic maturation through brain-derived neurotrophic factor signaling

Fig. 4.

BDNF increases the structural maturation and the lifetime of AZs in organotypic cultures. A Hippocampal organotypic cultures treated with buffer (-BDNF) on DIV5; treated with DMSO (control) on DIV6; fixed 6 h later; and immunostained for MAP2 (red), synaptophysin (green), and bassoon (blue). B Hippocampal organotypic cultures treated with BDNF (+BDNF) on DIV5; treated with DMSO (control) on DIV6; fixed 6 h later; and immunostained for MAP2 (red), synaptophysin (green), and bassoon (blue). C Hippocampal organotypic cultures treated with buffer (-BDNF) on DIV5; treated with latrunculin A (+LatA) on DIV6; fixed 6 h later; and immunostained for MAP2 (red), synaptophysin (green), and bassoon (blue). D Hippocampal organotypic cultures treated with BDNF (+BDNF) on DIV5; treated with latrunculin A (+LatA) on DIV6; fixed 6 h later; and immunostained for MAP2 (red), synaptophysin (green), and bassoon (blue). E Hippocampal organotypic cultures treated with buffer (-BDNF) on DIV5; treated with anysomycin (+Anisomycin) on DIV6; fixed 6 h later; and immunostained for MAP2 (red), synaptophysin (green), and bassoon (blue). F Hippocampal organotypic cultures treated with BDNF (+BDNF) on DIV5; treated with anysomycin (+Anisomycin) on DIV6; fixed 6 h later; and immunostained for MAP2 (red), synaptophysin (green), and bassoon (blue). G Quantification of the number of bassoon puncta per 100 μm2 for the conditions indicated in panels AF. Mean + SEM are shown; N = 3 experiments, n = 10 images per condition and experiment; two-way ANOVA with post hoc Sidak tests: interaction is significant (***p< 0.0001, F(2, 174)=38.43; ***p< 0.0001 for NT control vs. NT + LatA and for NT control vs. NT + Anisomycin; p> 0.05 for BDNF control vs. BDNF + LatA and for BDNF control vs. BDNF + Anisomycin). Scale bar is 10 μm

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