Fig. 7.

BDNF restores presynaptic maturation in NL1-deficient cultures. A, B Representative images and quantification of cultured cortical neurons from WT and NL1-KO mice fixed on DIV15. Prior to fixation, the neurons were treated with either BDNF for 22 h, LatA for 18 h, or both (see labeling on the left). Following fixation, the neurons were immunolabeled with a bassoon antibody (magenta). Mean + SEM; N = 3 experiments, n = 10 cells per condition and experiment; two-way ANOVA with post hoc Sidak tests: interaction is significant: ***p< 0.0001; F(3, 232)=95.61; ***p< 0.0001 for KO control vs. KO + LatA, for KO + BDNF vs. KO + LatA, and for KO control vs. KO + BDNF + LatA. Scale bar is 10 μm. C, D Representative images and quantification of cultured cortical neurons from WT and NL1-KO mice fixed on DIV15. On DIV15 cultures were stimulated in the presence of antibodies against the lumenal domain of synaptotagmin-1 (Syt1) to label the recycling of synaptic vesicles. After washing, the neurons were fixed and immunolabeled with secondary antibodies against the Syt-1 antibody (magenta). Mean ± SEM; N = 3 experiments, n = 10 cells per condition and experiment; two-way ANOVA with post hoc Sidak tests: interaction is significant: ***p< 0.0001; F(1, 116)=68.32; ***p< 0.0001 for WT control vs. KO control and for KO control vs. KO + BDNF. Scale bar is 10 μm