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Fig. 2 | BMC Biology

Fig. 2

From: Self-organising human gonads generated by a Matrigel-based gradient system

Fig. 2

Expression of basement membrane proteins in the 3-LGS. Immunolabelling of basement membrane proteins collagen 4 (COL4) and fibronectin (FN) (both red) highlight the compartmentalised testicular organoid structure (TO) (representative organoid image from 8 wpc embryonic tissue sample) and the lack of organisation observed in testicular mesonephric organoids (TMO) (representative organoid image from 7.5 wpc embryonic tissue sample) when compared to male (M) 8 wpc in vivo control. Collagen 4 immunostaining revealed the onset of early tissue organisation in ovarian organoids (OO) (representative organoid image from 10 wpc embryonic tissue sample). All organoid images from day 14 culture samples unless otherwise stated. Scale bars, 50 μm (insets, 10 μm)

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