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Fig. 3 | BMC Biology

Fig. 3

From: Self-organising human gonads generated by a Matrigel-based gradient system

Fig. 3

The 3-LGS supports the male somatic cell environment. A Immunolabelling of Sertoli cell marker SOX9 (red) and cytoplasmic anti-Müllerian hormone (AMH) (green) within newly formed cords in testicular organoid (TO) (representative organoid image from 8 wpc embryonic tissue sample). B Actin alpha 2 (ACTA2) (green) expressing vascular smooth muscle cells and peritubular myoid cells in TO (representative organoid image from 8 wpc embryonic tissue sample) and testicular mesonephric organoid (TMO) (representative organoid image from 7.5 wpc embryonic tissue sample). All organoid images from day 14 culture samples. In vivo control male (M) 8 wpc testis. Scale bars, 50 μm (insets, 10 μm)

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