Fig. 5
From: Self-organising human gonads generated by a Matrigel-based gradient system
The 3-LGS maintains germ cell survival in the female but not the male. Ovarian organoids (OO) support germ cell survival throughout culture as indicated by immunolabelling for both A DDX4 (primordial germ cell marker) (green) and B POU5F1 (pluripotency marker) (red) (representative organoid images from 10 wpc embryonic tissue sample). In vivo control female (F) 10 wpc ovary. No DDX4-positive cells were observed in testicular organoids (TO) (A); however, a limited number of POU5F1-positive cells (B) were detected (representative images from 8 wpc embryonic tissue sample). In vivo male germ cell distribution demonstrated in 8 wpc control. All images from day 14 culture samples. Scale bars, 50 μm (insets, 10 μm)