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Fig. 5 | BMC Biology

Fig. 5

From: PACmn for improved optogenetic control of intracellular cAMP

Fig. 5

PACmn raises cAMP in hippocampal neurons. A Sample currents elicited by a 50-ms 470 nm light pulse (arrow) in neurons expressing either bPAC(wt) or PACmn together with mCNG. B Photocurrent amplitude and C slope recorded in neurons expressing PACmn or bPAC(wt) together with mCNG in response to 50 ms 470-nm light pulses of varying intensity. D Time from stimulation to onset, peak, and time from the peak to decay to ½ peak of responses to 50 ms, 1 mW mm-2 470 nm light pulses. E Currents recorded from a neuron expressing PACmn together with mCNG in response to 50 ms, 1 mW mm-2 470-nm light pulses (arrows), before and 15 min after wash-in of forskolin (FSK; 100 μM) and IBMX (75 μM). Plotted are median and interquartile range

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