Fig. 1
Mapping of sex-peptide insensitive EMS allele QB62 to Nup54. A Schematic of the Nup54 chromosomal region depicting gene models and chromosomal deficiencies used below the chromosomal nucleotide positions. Coding parts are shown as black and non-coding parts as white boxes. Transposon insertions are show as triangles. The sequence of the deletion in Nup54QB62, and the promoter fragment used for genomic rescue construct gNup54 are shown below the gene model. B Receptivity of wild type and transheterozygous Nup54QB62/Df(2R)9B4, Nup54MB03363/Df(2R)9B4, and Nup54MB03363/Df(2R)9B4; gNup54 females after sex-peptide (SP, black) or Ringer’s (R, white) injection measured by counting mating females in a 1 h time period 3 h after SP or R injection, respectively. The means with the standard error for three or four experiments with 18–23 females each are shown, and statistically significant differences from ANOVA post hoc pairwise comparisons are indicated by different letters (a, b P≤0.05, c ns). C Oviposition of wild-type and transheterozygous Nup54QB62/Df(2R)9B4, Nup54MB03363/Df(2R)9B4, and Nup54MB03363/Df(2R)9B4; gNup54 females after sex-peptide (SP, black) or Ringer’s (R, white) injection shown as means of eggs laid in 18 h with the standard error for 10 females each, respectively, and statistically significant differences from ANOVA post hoc pairwise comparisons are indicated by different letters (P≤0.0001). D Number of stage 14 oocytes present in ovaries of sexually mature virgin wild type and transheterozygous Nup54QB62/Df(2R)9B4 and Nup54MB03363/Df(2R)9B4 females shown as means with the standard error for seven ovaries. E Ribbon diagram of the structure of the channel Nup complex consisting of Nup54, Nup58, and Nup62 with the position of the Mi{ET1}Nup54MB03363 transposon insertion indicated leading to a partially functional truncated protein. The data underlying the presented graphs are in Additional file 6