Fig. 2

Nup54 is required to establish neuronal projections of pickpocket neurons. A–F Representative larval ventral nerve cord expressing CD8::GFP from UAS by ppkGAL4 in wild type (A–C) and Nup54^QB62/Df(2R)9B4 (D–F). Magnified corresponding areas indicated in D are shown for wild-type (B and C) and Nup54^QB62/Df(2R)9B4 (E and F). Arrowheads indicate missing commissures or connectives. G–J Representative adult brain and ventral nerve cord (VNC) expressing CD8::GFP from UAS by ppkGAL4 in wild type (G and I) and Nup54^QB62/Df(2R)9B4 (H and J). Arrowheads point towards cell bodies of ppk expressing neurons with absent neuronal arborizations. K Representative adult brain expressing histone2B::YFP from UAS by ppkGAL4 in wild-type. Arrowheads point towards cell bodies of the four paired ppk expressing neurons in the adult brain. The scale bar in A is 50 μm and in K is 100 μm. L Representative adult brain expressing CD8::GFP only in head ppk neurons by the intersection of ppkGAL4/UAS FRTstopFRT CD8GFP expression with otdflp, which expresses only in the brain