Fig. 1

Morphotype and aggregation/adhesion of HeLa clones. A Morphology assessed by phase-contrast microscopy. Scale bars: 100 μm. B Cellular sociology of the different NDPK D clones. Clone partitions and graphs were obtained by three different methods after 24 h of culture [16]. Two parameters were deduced from each method, namely AD (area disorder) and RFH (roundness factor homogeneity) for Voronoi’s partition, m (average length) and σ (standard deviation) for both Delaunay’s graph and the MST. C Slow aggregation assay, performed by seeding the HeLa clones on top of a gelified agar medium. Scale bars: 200 μm. D N-cadherin levels. Representative immunoblots of HeLa cell extracts run in duplicate. Abbr. of HeLa clones throughout the text according to the expressed NDPK-D: CTR, control/empty vector; WT, wild-type; BD, CL-binding-deficient mutant; KD, kinase-dead mutant. Where indicated, two independently isolated clones of the same type (e.g., CTR1, CTR2) were analyzed