Fig. 4

Mitochondrial network structure of HeLa clones. Network parameters determined in HeLa cells harboring empty vector control (CTR) or expressing wild-type (WT) or mutant NDPK-D (BD, KD), fixed and immunostained for mitochondrial MnSOD. A Representative confocal images show the regions of interests used for quantification (faint line boxes) and a representative region (bold line box) shown with 3.5-fold magnification to the right. Scale bar: 20 μm. B Average length of the mitochondrial filaments. C Average area of the mitochondrial filaments. D Elongation factor of the mitochondtrial filaments. All data are means ± SEM (n=5). *p< 0.05 relative to control/empty vector (CTR); ^#p< 0.05 and ^##p< 0.01 relative to wild-type (WT). For clone abbreviations, see Fig. 1