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Fig. 2 | BMC Biology

Fig. 2

From: MIEF1/2 orchestrate mitochondrial dynamics through direct engagement with both the fission and fusion machineries

Fig. 2

Expression of MIEF1-V5 or MIEF2-V5 partially reverses mitochondrial fragmentation induced by knockdown of Mfn1, Mfn2, or OPA1. a–d Confocal images of mitochondrial morphology in 293T cells treated with Mfn1 (a), Mfn2 (b), Mfn1 plus Mfn2 (c), or OPA1 (d) siRNA, followed by transfection with empty vector, MIEF1-V5, or MIEF2-V5 as indicated. The cells were stained with MitoTracker (red) followed by immunostaining with anti-V5 (blue) antibody. e Percentages (mean ± SEM) of cells with indicated mitochondrial morphologies in 293T cells as shown in (ad) in three independent experiments for each condition (n represents the total number of cells analyzed for each condition)

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