Fig. 8
From: Circulating miRNA repertoire as a biomarker of metabolic and reproductive states in rainbow trout
Blood plasma abundance of candidate biomarker c-miRNAs. Quantitative PCR analysis of selected miRNAs during the reproductive cycle and in response to feeding rate. The following reproductive stages were analyzed: pre-vitellogenesis (PV), early vitellogenesis (EV), late vitellogenesis (LV), and ovulation (OV). The feeding rate was either ad libitum (al) or restricted (r). Significant differences (ANOVA) in expression levels (arbitrary units, a.u.) are indicated for reproductive stage (RS), feeding rate (FR), and feeding rate—stage interactions (RS X FR). *p<0.05, **p<0.05, n.s. not significant (p>0.05). Replicates (N= 5 or 6) correspond to different individual fish. Corresponding data are available in Additional file 6