Fig. 1

Single-molecule super-resolution imaging (dSTORM) of MTs in HeLa cells treated with colcemid reveals increasingly aberrant filament curvature. Cells were treated with colcemid at the specified concentrations for 5 h before fixation. Cells were immunolabelled for tubulin with Alexa Fluor 647, imaged and super-resolution images rendered using rapidSTORM. Increasingly abnormal curvatures and fragmentation of the MT architecture can be visually detected with significant losses observed at 100–200 nM. The yellow arrow in the 200-nM Col-treated cell indicates a MTOC. Images are representative of each treatment. N > 20 cells per condition. Scale bars = 10 μm