Fig. 2
Analysis of filament curvature induced by colcemid. a Filaments from dSTORM images of control and 80-nM colcemid-treated HeLa cells (left). Curvature analysis using SIFNE and coloured at each traced pixel for curvature between 0 and 2 rad/μm. SIFNE curvature output has been dilated by 10 pixels (using ImageJ) to enable visualization. Original SIFNE output shown in Additional file 1: Fig. S3. Scale bars = 1 μm. b Histogram of curvature data (curvature at each pixel) from a control cell and 80-nM colcemid cell with bins every 0.1 rad/μm. c Normalized exponential decays from histograms of control (N = 23) and 80-nM colcemid-treated cells (N = 25). d ν value of each cell analysed with SIFNE (N = 135 cells, 50.4 mm total filament length) with mean and standard error of means. All ν values were derived from exponential fits with adjusted R2 > 0.994. Parametric t-tests for each colcemid concentration against the control reveal a significant difference with 7 nM colcemid (**p = 0.0014) and each higher colcemid concentration 30–80 nM (****p < 0.0001). Data for d are provided in Additional file 3: Sheet 1