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Fig. 3 | BMC Biology

Fig. 3

From: Nanoscale characterization of drug-induced microtubule filament dysfunction using super-resolution microscopy

Fig. 3

Second-order SOFI produces optimal trade-off between sub-diffraction resolution and image clarity of live-cell MT filaments. a Representative diffraction-limited, 2nd- and 3rd-order SOFI images of MTs in a COS-7 cell labelled with MAP4-ffDronpa. Zoomed regions (dotted white box) show the same proximate filaments (yellow arrows) highlighting improved separation in the 2nd- and 3rd-order renderings, but increased loss of structure and clarity in the 3rd-order image. Scale bars = 5 μm (left-hand images) and 1 μm (right-hand images). b Intensity cross-sections across the proximate filaments showing improved resolution with 2nd- and 3rd-order SOFI. Values are FWHM of Gaussian fitted to the first peaks of the intensity plots. A Gaussian fitted to the first peak of the diffraction-limited trace (dotted line) resulted in a FWHM of 450 nm, demonstrating the poor image resolution. Data for b are provided in Additional file 3: Sheet 2

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