Fig. 4
Shorter SOFI integration times reduce smear caused by filament movement while retaining sub-diffraction resolutions. a First frames from SOFI time-lapse of live COS-7 MTs labelled with MAP4-ffDronpa and processed using different integration times (Itime) from 10 s to 11 min. Zoom region (dotted white box) from each Itime image showing the same filament feature indicated by yellow arrow (possibly more than 1 filament) having increasing width with increasing Itime. Scale bars = 5 μm (top row) and 1 μm (bottom row). b Intensity profile of filament feature width in a with different Itime and corresponding FWHM values after fitting with a Gaussian. c Filament feature width displacement through SOFI time-lapse with Itime = 20 s at t = 0, 2, 4 and 8 min