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Fig. 5 | BMC Biology

Fig. 5

From: Nanoscale characterization of drug-induced microtubule filament dysfunction using super-resolution microscopy

Fig. 5

Tracing filament activity from SOFI time-lapse enables sub-diffraction changes in filament length to be quantified. a, b Consecutive SOFI frames of Itime = 20 s showing sub-minute dynamics of a growing and b shrinking MT activity in HeLa cells. Yellow arrows indicate filament of interest. Scale bars = 1 μm. c Tracing a growing MT filament from a SOFI time-lapse. Green line measures the length from the reference base to the tip, except in the final frame of Itime = 20 s where filament becomes unclear. d Filament from c traced at each frame for 10 min with arrows indicating example measurements for both growth and shrinkage ∆length between successive frames

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