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Fig. 1 | BMC Biology

Fig. 1

From: Telomerase subunit Est2 marks internal sites that are prone to accumulate DNA damage

Fig. 1

Global occupancy of Est2 across the yeast genome. A The distribution of Est2 occupancy across the S. cerevisiae genome. Each triangle represents a non-telomeric binding site (NTBS) of Est2 on a chromosome. All the sites were present in at least 3 out of 5 independent experiments. Note, less triangles are visible on the cartoon because of the resolution of the graphic. Multiple regions that are located at close to each other or as clusters are depicted as one arrow. B MEME motif of NTBS regions. The binding sites displayed an enriched TG-richness similar to yeast telomeric regions. (E-value 1.1e−069) C ChIP-qPCR of four different NTBS regions (see Additional file 1: Table S1 for specification of the region). As a positive control, Est2-binding to telomere VI-R was plotted (Telo-VI-R). Reported ChIP values are normalized to input and ARO1 (non-telomeric control). Data are represented as mean ± standard error mean (SEM) of n = 5 biological replicates unless stated otherwise. Statistical significance was compared to ARO1 levels and determined using Student’s t-test. **p-value < 0.01 and ***p-value < 0.001

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