Fig. 2

Est2-binding to NTBS does not depend on Est1 and TLC1. ChIP analysis of Est1 and Est3 to four NTBS and one telomere (VI-R) (A, B). A Est1-binding to NTBS, ARO1, and Telo-VI-R regions. Bars represent enrichment over ARO1. Data are represented as mean ± SEM for n = 3 biological replicates. Statistical significance compared to untreated cells were determined using Student’s t-test. *p-value < 0.05 and **p-value < 0.01. B Est3-NTBS binding. C Est2-binding was monitored in synchronized cultures. For this, cells were synchronized using α-factor and released in the cell cycle. Binding was monitored every 15 min of release into the cell cycle. FACS analysis was performed to analyze the cell cycle stage of synchronized cells depicted in Additional file 4: Fig. S2A. The graph represents Est2-binding to telomere VI-R in wild type background (closed circles, in absence of TLC1 (open squares) and in the absence of Est1 (open triangles). D Est2-binding to NTBS #1-#4 in wild type background. E Representative data of Est2-binding to NTBS#1 in wild type, tlc1Δ, est1Δ (Additional file 4: Fig. S2B-D for NTBS #2 -#4). The data plotted are standard mean ± standard error for n = 3 replicates