Fig. 3

Est2 is recruited via an alternative pathway to NTBS. A Cdc13 was tagged internally and binding to four NTBS and telomere VI-R was monitored by ChIP-qPCR in asynchronous cultures (Additional file 5: Fig. S3A-B for synchrony ChIP-qPCR of Cdc13). B Ku70 is tagged internally and binding to NTBS and telomere VI-R was monitored by ChIP-qPCR in asynchronous cultures. ChIP values are normalized to input and ARO1 (non-telomeric control). Data are represented as mean ± SEM of n = 5 biological replicates unless stated otherwise. Statistical significance was compared to ARO1 levels and determined using Student’s t-test. **p-value < 0.01 and ***p-value < 0.001. C DNA Pol2 occupancy was monitored throughout the cell cycle to NTBS and telomeres. Representative data of DNA Pol2-binding to NTBS#1-4, normalized to input