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Fig. 4 | BMC Biology

Fig. 4

From: Zinc antagonizes iron-regulation of tyrosine hydroxylase activity and dopamine production in Drosophila melanogaster

Fig. 4

The in vivo effects of Catsup on TH appear primarily not due to TH phosphorylation and BH4 synthesis. A Sequence comparison among the fruit fly, human, and rat THs. Phosphorylation positions in mammalian TH and their corresponding amino acids in Drosophila TH are indicated with yellow arrowheads. Only the critical third phosphorylation site (Ser40) is conserved in the fly. Predicted metal-binding residues are marked with red arrows. B TH phosphorylation in SH-SY5Y cells under different metal or metal chelator treatments. No obvious and consistent changes were observed. An anti-phosphorylated TH antibody was used. Actin was used as a loading control. C The eclosion defect of Da-Gal4>GTPCH RNAi flies was rescued by DMPH4 supplementation in the food. Genotypes of the flies used were Da-Gal4>GTPCH RNAi. All values are presented as mean ± SEM; n = 6. ***p < 0.001. D DMPH4 supplementation in the food had no effect on the activity of TH. Genotype of the flies used was w1118. All values are presented as mean ± SEM; n = 6

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