Fig. 7

Modulation of zinc and iron homeostasis is an effective means to alter a spectrum of phenotypes associated with PD. A The mobility defect of PD flies (exposing to rotenone) could be rescued by Catsup RNAi, dZnT7 OE, or Mvl OE. Genotypes of the flies used were TH-Gal4>w¹¹¹⁸ (control), TH-Gal4>Catsup RNAi, TH-Gal4>Catsup OE, TH-Gal4>dZnT7 OE, and TH-Gal4>Mvl OE. All values are presented as mean ± SEM and included in Additional file 2: table S9; n ≥ 6. ***p < 0.001. B The shortened lifespan of PD flies (exposing to rotenone) could be modified by adjusting zinc transporter expressions. Genotypes of the flies used were TH-Gal4>w¹¹¹⁸ (control), TH-Gal4>Catsup RNAi, TH-Gal4>Catsup OE, TH-Gal4>dZnT7 OE, and TH-Gal4>Mvl OE. C The mobility rescue effect of Catsup RNAi on PD flies (exposing to rotenone) could be neutralized by hZIP7 expression. The exacerbated defect of Catsup OE on PD flies could be rescued by dZnT7 OE and Mvl OE. Genotypes of the flies used were TH-Gal4>w¹¹¹⁸ (control), TH-Gal4>Catsup RNAi, TH-Gal4>Catsup RNAi, hZIP7 OE, TH-Gal4>Catsup OE, TH-Gal4> Catsup OE;dZnT7 OE, TH-Gal4> Catsup OE, and Mvl OE. All values are presented as mean ± SEM and included in Additional file 2: table S10; n ≥ 6.. ***p < 0.001. One-way analysis of variance (ANOVA) was used for multiple groups. D The lifespan rescue of Catsup RNAi on PD flies (exposing to rotenone) could be neutralized by hZIP7 expression. The exacerbated defect of Catsup OE on PD flies could be rescued by dZnT7 OE and Mvl OE. Genotypes of the flies used were TH-Gal4>w¹¹¹⁸ (control), TH-Gal4>Catsup RNAi, TH-Gal4>Catsup RNAi, hZIP7 OE, TH-Gal4>Catsup OE, TH-Gal4> Catsup OE, dZnT7 OE, TH-Gal4> Catsup OE, and Mvl OE. All values are presented as mean ± SEM and included in Additional file 2: table S11; n ≥ 4. ***p < 0.001