Fig. 6

Corresponding elements of SecY and YidC serve similar mechanistic roles using similar amino acids. a SecY and YidC models aligned by fitting to a model membrane: G. thermodenitrificans SecY and substrate (6itc), M. jannaschii MJ0480 (predicted, see Additional file 2: Figure S4), and B. halodurans YidC2 (3wo7A). A cartoon substrate is superimposed on bacterial YidC to indicate the experimentally determined translocation interface and conformation [74]. YidC is shown clipped to allow a lateral view of the hydrophilic groove which would otherwise be occluded by h4h5, and likewise the plug loop in SecY N was removed. b Superposition of SecY and archaeal YidC (G. thermodenitrificans SecY N-half, 6itc, vs M. jannaschii MJ0480, 5c8j). Coloured segments correspond to the sequence logos in panel c. c Sequence logos for the structurally aligned regions of SecY and archaeal YidC. Column numbers correspond to the proteins in a