Fig. 1

Predicted isoform structure of LEA-1 and generation of a null allele and two endogenous fluorescent tags. Isoform annotations were combined from the UCSC genome browser (http://genome.ucsc.edu) from the February 2013 release (WBcel235/ce11) and Wormbase version WS278. Locations for the existing tm6452 indel mutation, the newly created CRISPR null mutation, and the insertion of mNeonGreen and mYPET fluorescent tags are indicated