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Fig. 2 | BMC Biology

Fig. 2

From: LEA motifs promote desiccation tolerance in vivo

Fig. 2

lea-1 mutants have a temperature-sensitive dauer formation defect. A Dauer larvae formed at 20 °C are resistant to 1% SDS. B Dauer larvae of lea-1Δ and lea-1(tm6452) mutants formed at 25 °C are sensitive to 1% SDS in both a wild-type (N2) background and a daf-2(e1370) mutant background. C lea-1Δ, lea-1(tm6452), daf-2;lea-1Δ and daf-2;lea-1(tm6452) mutant dauer-like larvae formed at 25 °C do not ingest fluorescent beads. Non-dauer larvae of each genotype did exhibit feeding behavior and ingest beads. D daf-2;lea-1Δ and daf-2;lea-1(tm6452) mutants at 25 °C have limited radial constriction relative to daf-2 mutant dauers. A one-way ANOVA indicates significant differences across genotypes (p < 0.0001) and a post-hoc Dunnett’s test indicates that both daf-2;lea-1Δ and daf-2;lea-1(tm6452) had increased width relative to daf-2 controls (p = 0.04, p < 0.0001, respectively). The width of dauer larvae was measured posterior to the pharynx. Points represent the width of individual worms and black bars depict the mean. The distribution of widths is shown above in density plots. E SEM images highlight the ultrastructure of alae of daf-2, daf-2;lea-1Δ and daf-2;lea-1(tm6452) mutants. daf-2 mutant dauers display normal alae consisting of five distinct ridges. Each of the lea-1 mutants have disrupted alae. F DIC images show daf-2;lea-1Δ and daf-2;lea-1(tm6452) dauer-like larvae with detached cuticles, as indicated by arrowheads

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