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Fig. 1 | BMC Biology

Fig. 1

From: Formation of nuclear condensates by the Mediator complex subunit Med15 in mammalian cells

Fig. 1

Distributions of Med1 nuclear foci in interphase and mitotic human cells. a Wide-field fluorescence images of a human U2OS cell nucleus stained with an anti-Med1 antibody. The z-interval between individual images is 0.3 μm. Scale bar: 5 μm. b Interphase and mitotic U2OS cells co-stained with an anti-Med1 antibody (green) and Hoechst33342 (blue). Scale bar: 10 μm. The yellow arrow indicates a cell undergoing mitosis. Insets 1 and 2 show the enlarged images of Med1 foci in interphase and mitotic cells, respectively. Scale bars: 5 μm. Similar results were obtained from three independent experiments. c The number of Med1 foci quantified in individual interphase or mitotic U2OS cells by AirLocalize program [22] (Intensity threshold: 450). The numbers of analyzed cells were 46 and 17, respectively. Student’s t test: p < 0.001 (indicated by ***)

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