Fig. 2

Med15 forms nuclear foci that are disrupted by Hexanediol treatment. a Fluorescence image of a U2OS cell transfected with AcGFP-hMed15. b Fluorescence images of U2OS cells stained with Hoechst33342 (blue) and an anti-Med15 antibody (green). Similar results were obtained from three independent experiments. c Fluorescence images of a human T24 cell line stably expressing GFP-hMed15 (green) and co-stained with an anti-Med1 antibody (red) and Hoechst33342 (blue). Yellow arrowheads indicate sites of colocalization. Similar results were obtained from three independent experiments. d Time-lapse fluorescence images of a cell from human T24 stable cell line expressing AcGFP-hMed15 during treatment with 0.5% 1, 6-Hexanediol and subsequent recovery. Yellow arrows indicate the time points of Hexanediol addition and withdrawal. Noted time points on the images are in mm:ss format. Similar results were obtained from three independent experiments. All scale bars: 5 μm