Fig. 3
From: Formation of nuclear condensates by the Mediator complex subunit Med15 in mammalian cells
Dynamics of Med15 nuclear foci in living cells. a Time-lapse images of an NIH3T3 cell nucleus expressing AcGFP-mMed15 during a FRAP experiment. Yellow boxes indicate the photobleached area. t = 0.00 s indicates the time point immediately after photobleaching. b Plot of GFP-mMed15 fluorescence intensity at the photobleached area within 42 s after photobleaching. Time intervals between individual frames in the first 20 cycles and the last 20 cycles of post-bleach were 655 ms and 5 s, respectively. Data are presented as the mean ± SEM, n = 3. c Time-lapse images of GFP-mMed15 foci that exhibited fusion and fission events (highlighted in yellow boxes and enlarged at upper-right insets). All scale bars: 5 μm