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Fig. 4 | BMC Biology

Fig. 4

From: Formation of nuclear condensates by the Mediator complex subunit Med15 in mammalian cells

Fig. 4

DYRK3 overexpression disrupts Med1 foci and Med15 foci. a, b Fluorescence images of U2OS cells transfected with mCherry (a) or mCherry-NLS*-DYRK3 (b) and stained with anti-Med1 (green). c, d Fluorescence images of human T24 cells that stably expressed GFP-Med15 (green) and were transfected with TagRFP (c) or TagRFP-NLS*-DYRK3 (d). All scale bars are 10 μm. In b and d, red arrowheads indicate the transfected cells. Similar results were obtained from three independent experiments. e The number of Med1 clusters quantified in individual U2OS cells after transfection with mCherry (n = 29) or mCherry-NLS*-DYRK3 (n = 44) (Intensity threshold: 600). Student’s t test: p < 0.001 (indicated by ***). f The percentage of cells displaying clusters of GFP-Med15 or diffuse localizations in the nucleoplasm after transfection with TagRFP (n = 28) or TagRFP-NLS*-DYRK3 (n = 57). Fisher’s exact test: p < 0.001 (indicated by ***)

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