Fig. 7

Testing the effects of Hexanediol treatment on IEG activation during serum response. a The experimental diagram. In Group I, NIH3T3 cells were serum starved for 24 h and then stimulated with growth media containing 20% serum. In Group II, cells were serum starved for 24 h, treated with 0.5% 1,6-Hexanediol for 1 min and then stimulated with media containing 20% serum but no Hexanediol. IEG expression was measured before adding serum (Group I), before and after Hexanediol treatment (Group II) and at distinct time points after adding 20% serum (Group I & II) by RT-qPCR. b–d Fold change of c-Fos (b), c-Jun (c), and Egr-1 (d) mRNA expression after serum stimulation in Group I cells (blue bars) and in 0.5% Hexanediol-treated cells (orange bars). Three experimental replicates were performed. In b–g, data are presented as mean ± SEM, and y-axes are plotted in log2 scale to facilitate comparison between time points. GAPDH mRNA expression was used for internal controls