Fig. 2

A screen for the clients regulated by the Hsc82-Ids2 chaperone complex. A A schematic diagram highlights the steps in the screening of the Hsc82-Ids2 clients. B Ids2 maintains the protein levels of four candidates in the ATP metabolic process. Overnight cells of the indicated strains were refreshed to OD = 0.5 and then transferred to 30 or 37 °C for 3 h. Total protein was extracted, and the western blot was hybridized with various antibodies. Pgk1 was served as a loading control. The numbers below are the means (n = 3) of the intensity ratios of HA/Pgk1 compared with that of the wild-type strain. C Ids2 does not control the stability of the other four candidates in the ATP metabolic process. Overnight cells of the indicated strains were refreshed to OD = 0.5 in 30 °C for 3 h. Western blotting was conducted as described above