Fig. 4

Ids2 interacts with Atp3 directly. A Co-immunoprecipitation assays were conducted using cells transformed with the pRS426-Ids2-Myc₁₃ and pRS313-Atp1-HA₃, pRS313-Atp2-HA₃, pRS423-Atp3-HA₃, or pRS426-Coa3-HA₃ plasmids. Lysates were precipitated by an HA antibody and co-precipitated proteins were detected by a Myc antibody (n = 3). B Talon-bead-bound recombinant His6-Atp1, Atp2, Atp3, or Coa3 proteins were incubated with recombinant GST-tagged Ids2. The pulldown products were detected by the indicated antibodies (n = 3). C Ten-fold serially diluted cells were grown on SC medium plates without tryptophan supplemented with 2% glucose or 3% glycerol. D Confocal microscopic images show the localization of Ids2. W303 cells containing pRS426-IDS2-GFP and pHS12-COX4-DsRed (a mitochondria marker) were grown in SC medium supplemented with 3% glycerol for 48 h. Filters selective for the green fluorescence of GFP (left) or the red fluorescence of DsRed (middle) were used. Green and red fluorescence pictures merged are shown (right)