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Fig. 1 | BMC Biology

Fig. 1

From: Co-option of the limb patterning program in cephalopod eye development

Fig. 1

Lentigenic cell differentiation and DpS-Crystallin expression in the squid. A Cartoon diagram of a squid embryo (anterior), en face cartoon of the developing eye (red dotted line shows cross-section plane throughout the paper), and developing lentigenic cells and lens (cartoon of lens and lentigenic cells based on [43]). B Cross-section of the developing anterior segment at Arnold stages 21 late, 23, 25, 27, and 29 identifying differentiation of lentigenic cells [49]. White: Sytox-Green labeling nuclei, Yellow: False-colored lentigenic cell nuclei corresponding to the LC2 population identified by nuclear morphology [43, 44, 46]. Blue is the outline of the lens, as identified using phalloidin staining (not shown). First evidence of LC2 cells is late stage 21. Lentigenic cell number multiplies and distribution grows across the anterior segment (as) throughout development. Scale is 50 μm. C In situ hybridization of DpS-Crystallin in whole-mount and cryo-section. Stage 19 is an anterior view, the boundary between the retina placode and the lip cells is highlighted with a dotted line. No DpS-Crystallin expression is apparent at this stage. Stages 21–27 are shown in a lateral view of the embryo on the left and a cross-section of the eye on the right. Anterior of the embryo is down in the sections. The retina is outlined with a dashed grey line in stage 21 and 23. DpS-Crystallin expression corresponds with LC2 population. Scale is 500 μm in whole mount images. Scale is 100 μm in sectioned images. as, anterior segment; a, arm; aco, anterior chamber organ; e, eye; f, funnel; LC2, lentigenic cell population; lp, lip; m, mantle; mo, mouth; rp, retina placode; r, retina; y, yolk. Red arrow highlights the lens

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