Fig. 2
Ability of Osh6p and ORP8 to transfer and exchange cellular PS and PI(4)P species. a Initial PS and PI(4)P transfer rates, measured along opposite directions, between LA and LB liposomes (200 μM total lipid each) with Osh6p (200 nM) at 30 °C, in the absence of counterligand or in a situation of lipid exchange, with diverse combinations of PS (16:0/18:1 or 18:0/18:1, 5% in LA liposomes) and PI(4)P species (16:0/16:0, 16:0/18:1 or 18:0/20:4, 5% in LB liposomes). Data are represented as mean ± s.e.m. (n = 3–4). b Similar experiments were conducted with ORD8 (240 nM) at 37 °C using 18:1/18:1-PI(4)P instead of 16:0/18:1-PI(4)P. Data are represented as mean ± s.e.m. (n = 3–4)