Fig. 5

Single-cell characterization of lncRNA expression in the Harpegnathos brain. A Number of genes whose exons cover the same, more, or fewer base pairs in HSAL51 compared to HSAL50, classified by biotype. B Scatter plot for UMI counts per gene in HSAL50 vs. HSAL51. LncRNAs are highlighted in dark gray, and gene of interest mentioned in subsequent panels is shown in black or red. C Heatmap showing normalized UMI counts of neuron-specific lncRNA LOC112589360 in HSAL50 and HSAL51 (top) and % of UMIs mapping to LOC11258930 in each cell type (bottom). D % of UMIs mapping to ensheathing glia-specific lncRNA LOC112590028 in HSAL50 (top) and HSAL51 (bottom). E Heatmap with normalized UMI counts of lncRNA LOC1125890028 (top) and protein-coding gene wdp (bottom). F Diagram showing relative positions of genes in the vicinity of wdp in Drosophila, Harpegnathos, and Apis mellifera. Orthologous genes are the same color. G Heatmap showing normalized UMI counts of wdp in single-cell RNA-seq from Drosophila brains [44]. All single-cell data were reanalyzed from [22]