Fig. 4

Anatomical and cell-type context of diel genes in female worms. A Heatmap of the 48 female diel genes that are single cell markers [25]. Each row represents a diel gene, ordered vertically by phase within anatomical systems containing constituent cell types. Diel genes that are markers for multiple cell type categories are placed within cell type (and their category) with the greatest difference between their Seurat pct1-pct2 scores. “Smp_” prefixes have been removed from gene identifiers for clarity; time of peak expression (acrophase) in parentheses (ZT = Zeitgeber time). p value of cell types significantly enriched in diel genes given in brackets [p < 0.05]. B Temporal relative expression profiles of Hsp90 (Smp_072330) and FKBP-type peptidylprolyl isomerase (PPIase) (Smp_004780) showing peaks of expression at night. C WISH expression of PPIase showing labelled transcripts in female worms in all oocytes in the ovary (scale bar = 100 μm) (100% of individuals examined, n = 10). D Female (paired) worms in vitro lay more eggs at night (dark phase) (median and interquartile ranges) than during the day (n = 12 female worms; median (night egg count − day egg count) = 12.0; paired Wilcoxon test: p = 0.003216)