Skip to main content
Fig. 1 | BMC Biology

Fig. 1

From: JNK signaling provides a novel therapeutic target for Rett syndrome

Fig. 1

JNK signaling activation in Mecp2y/- Bird male mice. a Growth curves of Mecp2y/- mice (n=25) compared to their wt littermates (n=25) from 3 to 7 weeks of age. b, c Behavioral analysis of Mecp2y/- mice (n=10) compared to their control wt (n=10) from 3 to 7 weeks of age in the Rotarod (b) and open field (c) tests (parameters shown: duration of immobility and distance moved). The distance moved by each experimental group was also presented in the arena plots under the open field graphs. d On-set and number of apnea in Mecp2y/- and wt mice at 6, 7, 8, and 9 weeks of ages in the table and the graphs, in the lower part, the representative plethysmographic traces of Mecp2y/− (n=15) vs wt (n=6) characterizing the respiratory patterns and breathing dysfunction. e JNK signaling pathway activation in the whole homogenate: Western blots and quantifications of P-c-Jun/c-Jun and P-JNK/JNK ratios in the cortex, hippocampus, and cerebellum of 7-week-old Mecp2y/- (n=10) and wt (n=10) mice. f Western blots and quantifications of TIF fraction (post-synaptic elements) showed the JNK activation in cerebellum of 7-week-old Mecp2y/- (n=10) compared to wt (n=10) mice. g Western blots and quantifications showed PSD alterations in Mecp2y/- (n=10) compared to wt (n=10) mice. Data were shown as mean ± SEM. Significance was calculated using two‐way ANOVA for repetitive measurements followed by Bonferroni post hoc test (panels a, b, c, and d) or Student’s t test followed by Tukey’s post hoc test (panels e, f, g). Statistical significance: *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001

Back to article page