Fig. 3

Maintenance of hepatic energetic homeostasis during short-term hypoxic stress is dependent on enhanced hepatic respiratory capacity. Targeted metabolomics performed using LC-MS, including presentation of dihydroxyacetone phosphate (DHAP), 2/3-phosphoglycerate (PG), acetyl CoA (AcCoA) and phosphocreatine (PCr). Data obtained from the peak area ratio, corrected to internal standards and protein concentration. For heatmap presentation, data was normalised using autoscaling and generalised logarithm transformation. n = 9 Veh normoxic, n = 8 all remaining groups. The 2 d hypoxic exposure with GSK932121A administration was defined as statistically significant across all metabolites presented through use of a two-way ANOVA plus false discovery rate correction (two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli, Q = 5%, threshold p value < 0.032), followed by a post-hoc Tukey’s test (p < 0.05)